Differential effects of melatonin on adipose tissues under normoestrogenic and estrogen-deficient conditions in rats

Melatonin effects on white and brown adipose tissues in rats

  • Danielle Aparecida Munhos Hermoso Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
  • Lenilson da Fonseca Roza Department of Zootechics, University of Maringá, Maringá 87020900, Brazil
  • Aparecida Pinto Munhos Hermoso Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
  • Eduardo Makiwama Klosowski Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
  • Franciele Neves Moreno Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
  • Maria Raquel Marçal Natali Department of Morphological Sciences, University of Maringá, Maringá 87020900, Brazil
  • Tatiana Carlesso Santos Department of Zootechics, University of Maringá, Maringá 87020900, Brazil
  • Jorgete Constantin Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
  • Rodrigo Polimeni Constantin Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
  • Eduardo Hideo Gilglione Signal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Brussels, B1070, Belgium
  • Emy Luiza Ishii Iwamoto Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil
DOI: https://doi.org/10.32794/mr112500175
Keywords: Melatonin, obesity, antioxidant, menopause, ovariectomy, oxidative stress, adipose tissue

Abstract

In post-menopause, oxidative stress due to the decline of natural antioxidants increases the susceptibility to metabolic syndromes (MetS). Estrogen and melatonin (MEL) share antioxidant properties; however, few studies have reported the impact of estrogen deficiency and MEL treatment on morphology, redox status, and antioxidant defense capacity of diverse adipose tissue (AT) subtypes. To investigate this issue, MEL was administered to ovariectomized (OVX) rats and sham-operated rats for 16 weeks (10 mg/kg). The adipocyte morphology, oxidative stress parameters and antioxidant enzyme activity were evaluated in the visceral retroperitoneal adipose tissue (rVAT), subcutaneous inguinal adipose tissue (iSAT) and brown adipose tissue (BAT). In OVX rats, MEL treatment suppressed rVAT hypertrophy and increased the prevalence of small adipocytes in iSAT, suggesting a better lipid distribution among ATs. MEL treatment increased glutathione reductase and glucose-6-phosphate dehydrogenase activity in iSAT; therefore, restored the glutathione level. In rVAT, MEL increased glutathione peroxidase and glutathione reductase activity. MEL minimized the risks for the development of metabolic abnormalities due to estrogen deficiency. However, under normoestrogenic condition, MEL decreased plasma estradiol levels and uterine mass, raising the concerning of its effect on reproductive functions.


Author Biographies

Lenilson da Fonseca Roza, Department of Zootechics, University of Maringá, Maringá 87020900, Brazil

Department of Zootechics

Aparecida Pinto Munhos Hermoso, Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil

Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis

Eduardo Makiwama Klosowski, Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil

Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis

Franciele Neves Moreno, Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil

Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis


Maria Raquel Marçal Natali, Department of Morphological Sciences, University of Maringá, Maringá 87020900, Brazil

3Department of Morphological Sciences

Tatiana Carlesso Santos, Department of Zootechics, University of Maringá, Maringá 87020900, Brazil

Department of Zootechics

Jorgete Constantin, Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil

Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis

Rodrigo Polimeni Constantin, Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis, University of Maringá, Maringá 87020900, Brazil

Department of Biochemistry, Laboratory of Biological Oxidation and Laboratory of Experimental Steatosis

Eduardo Hideo Gilglione, Signal Transduction and Metabolism Laboratory, Université Libre de Bruxelles, Brussels, B1070, Belgium

Signal Transduction and Metabolism Laboratory

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Published
2024-08-31
How to Cite
[1]
Hermoso, D.A.M., Roza, L. da F., Hermoso, A.P.M., Klosowski, E.M., Moreno, F.N., Marçal Natali, M.R., Santos, T.C., Constantin, J., Constantin, R.P., Gilglione, E.H. and Ishii Iwamoto, E.L. 2024. Differential effects of melatonin on adipose tissues under normoestrogenic and estrogen-deficient conditions in rats. Melatonin Research. 7, 2 (Aug. 2024), 187-212. DOI:https://doi.org/https://doi.org/10.32794/mr112500175.
Issue
Vol 7 No 2 (2024): Melatonin Research
Section
Research Articles
DOI
https://doi.org/10.32794/mr112500175
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