Pineal melatonin deprivation alters the mRNA expression of melatonin and steroidogenic-related receptor genes in rat oviduct and uterus during the estrus stage of estrous cycle
Melatonin and steroidogenic-related receptors in uterus
Keywords:
melatonin, pinealectomy, steroid, receptors, uterus, oviduct
Abstract
The effects of pinealectomy and melatonin replacement therapy on the mRNA expression of melatonin (Mt1, Mt2 and Rora), steroidogenic (Era - Estrogen a, Erb - Estrogen b, Ar - Androgen, Pgr - Progesterone, Lhr - LH and Oxr - Oxytocin receptors) -related receptors and melatonin-synthetic enzyme genes (Tph1, Asmt and Aanat) in oviduct and uterus of Wistar rats were studied. Tissues were collected from sham-pinealectomized (Control), pinealectomized (Pinx) and Pinx plus melatonin- supplemented (Pinx-Mel) females during the estrus stage of both the light (ZT6) and dark (ZT18) phases of the light-dark cycle. In oviduct, Pinx altered the mRNA expression of Mt1, Mt2, Rora, Era, Pgr, Oxr, Asmt and Aanat genes. In uterus, Pinx altered the mRNA expression of Mt1, Mt2, Rora, Lhr, Pgr, Oxr and Tph1 genes. Melatonin treatment partially or completely restored the Mt2, Rora, Era, Oxr, Asmt and Aanat mRNA expressions in oviduct and the Mt1, Mt2 and Rora mRNA expressions in uterus. These alterations varied according to the phase of light-dark cycle. The results suggest that pineal melatonin regulates the daily mRNA expression of melatonin and steroidogenic-related receptors in the rat oviduct and uterus during the estrus stage of estrous cycle. Melatonin also regulates the daily mRNA expression of Asmt and Aanat in oviduct and Tph1 in uterus.
References
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7. Tamura H, Nakamura Y, Korkmaz A, Manchester LC, Tan D-X, Sugino N, Reiter RJ (2009) Melatonin and the ovary: physiological and pathophysiological implications. Fertil. Steril. 92: 328-343.
8. Reiter RJ, Tamura H, Tan D-X, Xu X-Y (2014) Melatonin and the circadian system: contributions to successful female reproduction. Fertil. Steril. 102: 321-328.
9. Reiter RJ, Tan D-X, Korkmaz A, Rosales-Corral AS (2014) Melatonin and stable circadian rhythms optimize maternal, placental and fetal physiology. Hum. Reprod. Update. 20: 293-307.
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12. Kim MK, Park EA, Kim HJ, Choi WY, Cho JH, Lee WS, Cha KY, Kim YS, Lee DR, Yoon TK (2013) Does supplementation of in-vitro culture medium with melatonin improve IVF outcome in PCOS? Reprod. Biomed. Online 26: 22-29.
13. Tamura H, Takasaki A, Miwa I, Taniguchi K, Maekawa R, Asada H, Taketani T, Matsuoka A, Yamagata Y, Shimamura K, Morioka H, Ishikawa H, Reiter RJ, Sugino N (2008) Oxidative stress impairs oocyte quality and melatonin protects oocytes from free radical damage and improves fertilization rate. J. Pineal Res. 44: 280-287.
14. Tamura H, Jozaki M, Tanabe M, Shirafuta Y, Mihara Y, Shinagawa M, Tamura I, Maekawa R, sato S, Taketani T, Takasaki A, Reiter RJ, Sugino N (2020) Importance of melatonin in assisted reproductive technology and ovarian age. Int. J. Mol. Sci. 21: 1135. doi:10.3390/ijms21031135.
15. Chiba A, Akema T, Toyoda J (1994) Effects of pinealectomy and melatonin on the timing of proestrous luteinizing hormone surge in the rat. Neuroendocrinology 59: 163-168.
16. Cagnacci A, Soldani R, Yen SSC (1995) Exogenous melatonin enhances luteinizing hormone levels of women in the follicular but not in the luteal menstrual phase. Fertil. Steril. 68: 996-999.
17. Schlabritz-Loutsevitch N, Hellner N, Middendorf R, Muller D, Olcese J (2003) The human myometrium as a target for melatonin. J. Clin. Endocrinol. Metab. 88: 908-913.
18. Woo MMM, Tai C-J, Kang SK, Nathwani PS, Pang SF, Leung PCK (2011) Direct Action of Melatonin in Human Granulosa-Luteal Cells. J. Clin. Endocrinol. Metab. 2086: 4789-4797.
19. He C, Wang J, Zhang Z, Yang M, Li Y, Tian X, Ma T, Tao J, Zhu K, Song Y, Ji P, Liu G (2016) Mitochondria synthesize melatonin to ameliorate its function and improve mice oocyte’s quality under in vitro conditions. Int. J. Mol. Sci. 17: 939. doi:10.3390/ijms17060939.
20. Coelho LA, Peres R, Amaral FG, Reiter RJ, Cipolla-Neto J (2015) Daily differential expression of melatonin-related genes and clock genes in rat cumulus-oocyte complex: Changes after pinealectomy. J. Pineal Res. 58: 490-499.
21. Acuña-Castroviejo D, Escames G, Venegas C, Díaz-Casado ME, Lima-Cabello E, López LC, Rosales-Corral S, Tan D-X, Reiter RJ (2014) Extrapineal melatonin: sources, regulation, and potential functions. Cell. Mol. Life Sci. 71: 2997-3025.
22. Coelho LA, Buonfiglio DC, Kuwabara WMT, Andrade-Silva J, Gomes PRL, Scialfa JH. Peres R, Amaral FG, Cipolla-Neto J (2020) Melatonin regulates the expression of Bone Morphogenetic Protein 15 (Bmp-15), Growth Differentiation Factor 9 (Gdf-9) and LH receptor (Lhr) genes in developing follicles of rats. Melatonin Res. 3: 515-533, doi: 10.32794/mr11250076.
23. He C, Wang J, Li Y, Zhu K, Xu Z, Song Y, Song Y, Liu G (2015) Melatonin-related genes expressed in the mouse uterus during early gestation promote embryo implantation. J. Pineal Res. 58: 300-309.
24. Tamura H, Takasaki A, Taketani T, Tanabe M, Kizuka F, Lee L, Tamura I, Maekawa R, Asada H, Yamagata Y, Sugino N (2012) The role of melatonin as an antioxidant in the follicle. J. Ovarian Res. 5: 5. doi:10.1186/1757-2215-5-5.
25. Reiter RJ, Tan D-X, Terron MP, Flores LJ, Czarnocki Z (2007) Melatonin and its metabolites: new findings regarding their production and their radical scavenging actions. Acta Biochim. Pol. 54: 1-9.
26. Soares Jr JM, Masana MI, Ersahinn Ç, Dubocovich ML (2003) Funcional melatonin receptors in rat ovaries at various stages of estrous cycle. J. Pharmacol. Exp. Ther. 306: 694-702.
27. Seithikurippu R, Pandi-Perumal R, Trakht I, Srinivasan V, Spence DW, Maestroni GJM, Zisapel N, Cardinali DP (2008) Physiological effects of melatonin: Role of melatonin receptors and signal transduction pathways. Prog. Neurobiol. 85: 335-353.
28. Becker-André M, Wiesenberg I, Schaeren-Wiemers N, André E, Missbach, M, Saurat J-H, Carlberg C (1994) Pineal gland hormone melatonin binds and activates an orphan of the nuclear receptor superfamily. J. Biol. Chem. 269: 28531-28534.
29. Coelho LA, Andrade-Silva J, Motta-Teixeira LC, Amaral FG, Reiter RJ, Cipolla-Neto J (2019) The absence of pineal melatonin abolishes the daily rhythm of Tph1 (Tryptophan Hydroxylase 1), Asmt (Acetylserotonin O-Methyltransferase) and Aanat (Aralkylamine N- Acetyltransferase) mRNA expressions in rat testes. Mol. Neurobiol. 56: 7800-7809.
30. Paccola, CC, Resende CG, Stumpp T, Miraglia SM, Cipriano I (2013) The rat cycle revisited: a quantitative and qualitative analysis. Anim. Reprod. 10: 677-683.
31. Hoffman RA, Reiter RJ (1965) Rapid pinealectomy in hamsters and other small rodents. Anat. Rec. 153: 19-21.
32. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25: 402-408.
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Published
2022-04-05
How to Cite
[1]
Coelho, L.A., Gomes, P.R., Scialfa, J.H., Maganhin, C.C., Soares Jr., J.M., Amaral, F.G., Reiter, R. and Cipolla-Neto, J. 2022. Pineal melatonin deprivation alters the mRNA expression of melatonin and steroidogenic-related receptor genes in rat oviduct and uterus during the estrus stage of estrous cycle. Melatonin Research. 5, 1 (Apr. 2022), 68-83. DOI:https://doi.org/https://doi.org/10.32794/mr112500121.
Section
Research Articles
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