​Please cite this paper as:

Souza, E.S., Santos, A.A., Ribeiro-Paz, E.E., Córdoba-Moreno, M., Trevisan, I.L., Caldeira, W., Muxel, S.M., Sousa, K.D. and Markus, R.P. 2022. Melatonin synthesized by activated microglia orchestrates the progression of microglia from a pro-inflammatory to a recovery/repair phenotype. Melatonin Research. 5, 1 (Mar. 2022), 55-67. DOI:https://doi.org/https://doi.org/10.32794/mr112500120.

                                     SUPPLEMENTARY MATERIAL

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Supplementary Fig. 1. Representative histogram and microphotographs obtained by flowsight cytometry.

     Histogram of a selected sample showing 10.7% of CD11b/c+ cells gated from total nucleated cells (PI+/CD11b/c+). On the right, microphotographs obtained by flowsight cytometry show protein expression by fluorescence emission of the markers CD11b/c+ (green), PI (blue), and overlapping channels (MERGE). Protein expression of microglial activation markers IBA-1, NOS-2 and ARG-1 were analyzed according to this template in PI+/ CD11b/c+ population.

Table 1. Material, reagents, drugs and antibodies.

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