Please cite this paper as:

Ko, S.-F., Huang, T.-H., Lin, Y.-P., Chen, Y.-L. and Yip, H.-K., 2022. Accuracy and precision of 31P-MRS assessment for evaluating the effect of melatonin-pretreated mitochondria transferring on liver fibrosis of rats. Melatonin Research. 5, 1 (Mar. 2022), 18-33. DOI:https://doi.org/https://doi.org/10.32794/mr112500117.

Supplemental materials

Accuracy and precision of ³¹P-MRS assessment for evaluating the effect of melatonin-pretreated mitochondria transferring on liver fibrosis of rats

Supplemental Fig. 1. Assessment of oxygen consumption rate (OCR) in isolated mitochondria exposed to melatonin using a Seahorse XF24 Extracellular Flux Analyzer.

     Liver cells were exposed sequentially to oligomycin, FCCP, and rotenone/antimycin A. The arrows indicated time of addition of mitochondrial inhibitors. Simultaneous measurements of OCR were performed in triplicate samples of untreated (blue line) and treated (turquoise line) liver cells.