Melatonin and the heart circadian clock of euglycemic and type 2 diabetic male rats: a transcriptional evaluation

Transcriptional effects of melatonin in the rat heart

  • Jose Sinesio-Jr Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Paula Bargi-Souza 1Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil and 2Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Minas Gerais, MG, Brazil
  • Raphael A Matos Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Eduardo Almeida Leite Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Daniella Carmo Buonfiglio Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Jessica Andrade-Silva Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Livia Motta-Teixeira Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Rui Curi Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil and Post-graduate Interdisciplinary Program in Health Sciences, Institute of Physical Activity and Sports Sciences, Cruzeiro do Sul University, São Paulo, Brazil.
  • Martin Young Division of Cardiovascular Diseases, Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA
  • Jose Cipolla-Neto Department of Physiology and Biophysics, Institute of Biomedical Sciences-I, University of São Paulo, São Paulo, SP, Brazil
  • Rodrigo Antonio Peliciari-Garcia Morphophysiology & Pathology sector, Department of Biological Sciences, Federal University of São Paulo, Diadema, SP, Brazil
DOI: https://doi.org/10.32794/11250035
Keywords: Melatonin, pineal gland, cardiac tissue, clock gene, metabolism, Goto-Kakizaki rats

Abstract

Diabetes increases risk of various comorbidities, including retinopathy, neuropathy, and cardiovascular disease, comprising both ischemic and non-ischemic cardiomyopathy. Cardiac dysfunction during diabetes is associated with perturbations at histologic, metabolic, biochemical and molecular levels. The circadian clock is misaligned in multiple organs during diabetes, including the heart. Such alterations in clock function have been postulated to play a causal role in cardiac dysfunction even though the mechanisms leading to circadian misalignment are currently unknown. Melatonin has been reported to alter heart circadian clock components and its circulating levels are decreased during diabetes. These observations led to the hypothesis that decreased melatonin levels during diabetes could be related to mismanagement of the heart clock. To evaluate this hypothesis, in the current study male Wistar and non-obese type 2 diabetic Goto-Kakizaki (GK) rats were given melatonin supplementation in their drinking water during the dark phase (for 12-wks), followed by assessment of clock component and the mRNA expression of the clock-controlled genes in the hearts of these animals. Melatonin supplementation significantly altered mRNA expression of targeted genes in both euglycemic and diabetic rat hearts. Collectively, under the condition of diabetes, the jeopardized pineal melatonin synthesis with misalignment of cardiac circadian clock components may likely mediate heart metabolic dysfunction, and/or even cause cardiovascular diseases.


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Published
2019-08-31
How to Cite
[1]
Sinesio-Jr, J., Bargi-Souza, P., Matos, R., Leite, E., Buonfiglio, D., Andrade-Silva, J., Motta-Teixeira, L., Curi, R., Young, M., Cipolla-Neto, J. and Peliciari-Garcia, R.A. 2019. Melatonin and the heart circadian clock of euglycemic and type 2 diabetic male rats: a transcriptional evaluation. Melatonin Research. 2, 3 (Aug. 2019), 139-151. DOI:https://doi.org/https://doi.org/10.32794/11250035.
Issue
Vol 2 No 3 (2019): Melatonin Research
Section
Research Articles
DOI
https://doi.org/10.32794/11250035
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